FIG 6.

Laccase was involved in cellular oxidative stress resistance of C. cinerea during interspecific interaction with Gongronella sp. w5. C. cinerea exhibited higher ROS level (A) and H₂O₂ content (B) in lcc9 silencing strains compared to the wild-type strain after separated coculturing for 48 h in SAHX with sucrose with Gongronella sp. w5 sealed in sterile dialysis tubes. (C, D) The lcc9 silencing strains showed higher ROS level (C) and less biomass (D) than the wild-type strain in separated cocultures when exposed to 100 mM H₂O₂, 2 mM guaiacol, and 0.5 mM 3, 5-dihydroxynaphthalene. Chemicals were added at 36 h of separated cocultivation and cocultures were incubated for further 36 h. The data were analyzed using Student's t test (*, P < 0.05; **, P < 0.01; ***, P < 0.001). Data show mean ± SD, n = 3.