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Nonenzymatic conversion of unsaturated glucuronic acid monomers. a) Proposed reaction scheme. b) Degradation kinetics of the six glucuronan lyases. A235 was measured continuously at 40°C and pH 5 with 8 mM glucuronan, 100 mM NaCl, 2 mM CaCl and under otherwise standard assay parameters. c) 1H NMR spectrum of 24h time series for TpPL8A reaction at pH 4 and 30°C. d) 1H NMR spectrum of 24h time series for TpPL38A reaction at pH 6 and 30°C.
