FIG 4.

Maximum likelihood phylogenetic trees of protein sequences from the four PL families: a) PL7 family, b) PL20 family, c) PL38 family, and d) PL8 family. CUPP clusters are shown by the colored strips preceding the species names. All sequences were subjected to redundancy, fragmentation and alignment checks. All six lyases from T. parareesei were similar (90–100%) to T. reesei sequences (orthologous). For that reason the T. reesei sequences were omitted during the redundancy check. Characterized members are highlighted by symbols at the node ending and subfamily annotations are highlighted by the colored ellipsis next to the species names. Branch numbers indicate bootstrap values above 50.