TABLE 2.
Oligonucleotide primers used for PCR amplification and sequencing of strain 1 Enteric Group 137 16S rDNA
| No. | IDc | Sequence (5′–3′)a | Positionb | Orientation |
|---|---|---|---|---|
| 1 | F24 | GAGTTTGATYMTGGCTCAG | 9–27 | Forward |
| 2 | F23 | ACGGGAGGCAGCAGY | 344–358 | Forward |
| 3 | F22 | RCTGCTGCCTCCCGT | 344–358 | Reverse |
| 4 | F15 | TTACCGCGGCTGCTG | 519–533 | Reverse |
| 5 | F16 | TAGATACCCYGGTAGTCC | 789–806 | Forward |
| 6 | F17 | CCGTCWATTCMTTTGAGTTT | 907–926 | Reverse |
| 7 | F18 | GCAACGAGCGCAACC | 1099–1113 | Forward |
| 8 | F20 | CCATTGTARCACGTGTG | 1226–1242 | Reverse |
| 9 | F25 | AAGGAGGTGWTCCARCC | 1525–1541 | Reverse |
Base codes are standard International Union of Biochemistry codes for bases and ambiguity.
Numbering based on E. coli.
ID, identification.