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. Author manuscript; available in PMC: 2022 Jan 11.
Published in final edited form as: Acc Chem Res. 2020 Dec 31;54(2):388–402. doi: 10.1021/acs.accounts.0c00621

Figure 5.

Figure 5.

Detection of small molecules and monitoring enzyme activity using SiMREPS. (A) Experimental scheme showing the use of SiMREPS to probe the state of an aptamer for the high-sensitivity detection of small molecules by TIRF-microscopy. (B) Representative intensity-versus-time traces in the absence and presence of adenosine (50 pM). (C) Histograms of Nb+d in the absence (gray) or presence (cyan) of adenosine (50 pM). (D, E) Standard curve (D) and selectivity (E) of adenosine detection. A-E reproduced with permission from ref 16, Copyright 2019, American Chemical Society. (F) Experimental scheme for the detection of telomerase activity at the single-molecule level using SiMREPS. (G) Single-molecule kinetic traces in the presence (top) and absence (bottom) of telomerase activity. (H) The single-molecule assay showed a response in the presence of telomerase but not for other proteins. F-H reproduced with permission from ref 65, Copyright 2017, American Chemical Society.