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. 2021 Feb 9;118(1):169–183. doi: 10.1093/cvr/cvab044

Figure 3.

Figure 3

Transgenic cardiomyocyte GRK5 overexpression impacts cardiac remodeling and survival in ischemic heart failure. Representative TTC staining of hearts at 24 h post-myocardial infarction (MI) of NLC and TgGRK5 groups (A) as well as quantification of infarct size (% of LV) (B) (n = 5–7). TUNEL quantification of images of left ventricular (LV) tissue from NLC and TgGRK5 mice at remote area (RA), border zone (BZ), and infarct area at 24 h post-MI (C) (n = 6–7). Representative TUNEL staining (red) of LV tissue from NLC and TgGRK5 mice at the BZ counterstained with Troponin T (green) to visualize cardiomyocytes, at 4-days post-MI; DAPI was used as a nuclear marker (blue) (D). Scale bar in white (500 μm). TUNEL quantification of images of LV tissue from NLC and TgGRK5 mice at the RA, BZ, and infarct area, at 4-days post-MI (E) (n = 6–12). Representative images (F) and quantitative data (G) showing the percentage of fibrosis via Picrosirius red staining in LV tissue from NLC and TgGRK5 at 8-weeks post-Sham operation or MI (n = 5–8). Scale bar in black (500 μm). Quantification of RT-PCR data showing fold change of NLC-Sham for Col-1 (H), Col-3 (I), CTGF (J) and MMP-2 (K) in LV samples collected at 8-weeks post-surgery (n = 10–18). Kaplan–Meier survival curves of Sham and MI groups (L) (n = 8–89). Log-rank (Mantel–Cox) test has been used between groups. *P < 0.05, **P < 0.01, ****P < 0.0001, #P < 0.0001 vs. NLC-Sham, TgGRK5-Sham and NLC-MI RA and P < 0.001 vs. TgGRK5-MI RA, ##P < 0.0001 vs. NLC-Sham, TgGRK5-Sham, NLC-MI RA and TgGRK5-MI RA. One-way ANOVA with Tukey’s post hoc test, t-test or Mann–Whitney test were used between groups.