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. Author manuscript; available in PMC: 2023 Feb 1.
Published in final edited form as: Nitric Oxide. 2021 Dec 4;119:9–18. doi: 10.1016/j.niox.2021.12.002

Figure 4: Effects of TSE on Cygb expression in VSMCs.

Figure 4:

Cells were treated with 1% tobacco smoke extract (TSE) for 4, 24 and 48 hr and then whole cell lysate was blotted against Cytoglobin (Cygb) and β-actin antibodies. A, western blots for Cygb and β-actin in VSMCs, after treatment with TSE for 4, 24 and 48 hr. B, quantitation of Cygb expression from densitometry of the observed bands normalized for the β-actin loading control. Data shown are means ± SEM of 4 independent experiments. *: Denotes significant difference from untreated control at p < 0.001; #: Denotes significant difference from the TSE treated value at 4 hr at p < 0.001.