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. Author manuscript; available in PMC: 2023 Feb 1.
Published in final edited form as: Nitric Oxide. 2021 Dec 4;119:9–18. doi: 10.1016/j.niox.2021.12.002

Figure 6: Effect of Cygb-knockdown on NO metabolism by VSMCs.

Figure 6:

Cells were treated with Cytoglobin (Cygb) siRNA or scrambled RNA (ScrRNA) and then harvested for immunoblotting and measurement of nitric oxide (NO) metabolism. A, B, western blots and quantitation for Cygb and β-actin in control (CT), ScrRNA and Cygb-siRNA treated (Cygb-KD) VSMCs, respectively. C, the measured NO concentration over time after addition of NO in control, Scr-RNA and Cygb-KD treated VSMCs. D, shows the mean ± standard error of the NO metabolism rate (VNO), detected in control, Scr-RNA and Cygb-KD treated VSMCs, represented as % of the value in control cells. Each value represents the mean of 4–5 independent experiments ± SEM. A cell density of 3.5 × 106 VSMCs per ml was used in all groups. NO was injected as in figure 2. *: Denotes significant difference from control at p < 0.001.