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. Author manuscript; available in PMC: 2023 Feb 1.
Published in final edited form as: Nitric Oxide. 2021 Dec 4;119:9–18. doi: 10.1016/j.niox.2021.12.002

Figure 7: Effect of Cygb knockdown on NO metabolism by TSE-exposed VSMCs.

Figure 7:

Control non-transfected (CT), Scr-RNA pretreated and Cygb-siRNA pretreated (Cygb-KD) cells were untreated (UT) or treated with 1% tobacco smoke extract (TSE) for 48 hr and then harvested for measurement of nitric oxide (NO) metabolism. A, NO metabolism was measured as in Figure 2. Cell density of 3.5 × 106 VSMCs per ml was used in all groups. B, the mean ± standard error of the NO metabolism rate, VNO, from repeat experiments performed as in A. Values are shown for UT and TSE treated CT, Scr-RNA and Cygb-KD VSMCs with values depicted as % of untreated CT. Similar to figure 2, VNO in untreated cells was 10.9 ± 0.5 nMs−1. Each value represents the mean of 4–5 independent experiments ± SEM. *: Denotes significant difference from the UT control cells at p < 0.01. #: Denotes significant difference of each type of TSE-treated cells with respect to the corresponding untreated cells at p < 0.01.