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. Author manuscript; available in PMC: 2023 Feb 1.
Published in final edited form as: Nitric Oxide. 2021 Dec 4;119:9–18. doi: 10.1016/j.niox.2021.12.002

Figure 9: Effect of Cygb-KD on O2•− production in VSMCs.

Figure 9:

Confluent cells were treated with 1% of TSE for 48 hours, with or without pre-incubation with SODm and then incubated with DHE and DAPI and visualized by confocal fluorescence microscope. A, representative images of VSMCs from untreated (UT) and TSE treated Cygb knock down (Cygb-KD) or control scr-RNA treated VSMCs. B, quantitation of fluorescence intensity in VSMCs from a series of repeat experiments. Data shown are means ± SEM of 4 independent experiments. *: Denotes significant difference from corresponding untreated VSMCs at p < 0.001. #: Denotes significant difference of TSE-treated cells with respect to the corresponding Scr-RNA-treated cells at p < 0.001.