Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2022 Jan 11;13(1):51. doi: 10.1038/s41419-022-04500-w

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Fig. 1 — A RT-qPCR showed that linc01133 expression was upregulated in gastric cancer tissues compared to that in the normal gastric tissues (Mann–Whitney test, p = 0.048). B The expression of linc01133 was upregulated in gastric cancer cells compared to the immortalized normal gastric epithelial cells. C Online tool GEPIA analysis of linc01133 expression combining the TCGA and GTEx database. D, E GEPIA analysis of disease-free survival and overall survival of gastric patients with high (n = 192) and low (n = 192) linc01133 expression. F Schematic illustration of the luciferase vectors containing linc01133 promoter regions. G Luciferase assay confined the core regulatory regions to −502~−265 bp and −72~ +76 bp up-stream of transcriptional start site. H Luciferase assay showed that co-expression of c-JUN and c-FOS significantly increased the pGL3-502 luciferase activity. I Co-expression of c-JUN and c-FOS enhanced linc01133 expression in HEK293T, HGC27, MKN45 and SGC7901 cells but not in AGS cells. J JUN expression in gastric cancer cell lines. K Schematic illustration of the c-JUN-binding sites and the sites of primer pair design for ChIP assay. L–N ChIP-qPCR analyses showed that the promoter amplicons in the JUN-binding sites were significantly enriched by anti-Jun antibody. O, P The expression of Linc01133 was positively correlated with that of c-JUN and c-FOS in gastric cancer tissues (n = 25). Q Representative staining patterns of c-Jun and c-FOS detected by immunohistochemistry in gastric cancer tissues. Each experiment was performed in triplicates. Data are shown as mean ± SD, *P < 0.05, **P < 0.01.