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. 2022 Jan 11;13:261. doi: 10.1038/s41467-021-27901-5

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 6 — a Representative immunofluorescence staining of somatostatin (SST, green) and chromogranin A (CHGA, magenta) in enteroids treated with G14, G2D12, RSP, AS, AS→RSP, and AS→RASP. DNA (4′,6-diamidino-2-phenylindole (DAPI), blue). Scale bar = 50 µm. b Percentage of enteroids with SST staining in G14, G2D12, RSP, AS, AS→RSP, and AS→RASP treatments. Average results are from three different enteroid lines or passages. **p = 0.0017 (G14 to RSP, G2D12 to RSP), 0.0029 (G14 to AS, G2D12 to AS); ***p = 0.0004 (G14 to AS→RSP, G2D12 to AS→RSP), 0.0006 (G14 to AS→RASP, G2D12 to AS→RASP). c Representative immunofluorescence staining of serotonin (5HT, green) and CHGA (magenta) in enteroids treated with G14, G2D12, RSP, AS, AS→RSP and AS→RASP. DNA (DAPI, blue). Scale bar = 50 µm. d Percentage of enteroids with 5HT staining in G14, G2D12, RSP, AS, AS→RSP and AS→RASP treatments. Average results are from three different enteroid lines or passages. *p = 0.0183 (RSP to AS), 0.0134 (RSP to AS→RASP); **p = 0.0014; ***p = 0.0009; ****p < 0.0001. e Representative immunofluorescence staining of glucose-dependent insulinotropic peptide (GIP, green) and CHGA (magenta) in enteroids treated with G14, G2D12, RSP, AS, AS→RSP, and AS→RASP. DNA (DAPI, blue). Scale bar = 50 µm. f Percentage of enteroids with GIP staining in G14, G2D12, RSP, AS, AS→RSP, and AS→RASP treatments. Average results are from three different enteroid lines or passages. ***p = 0.0001; ****p < 0.0001. g Representative immunofluorescence staining of cholecystokinin (CCK, green) and CHGA (magenta) in enteroids treated with G14, G2D12, RSP, AS, AS→RSP, and AS→RASP. DNA (DAPI, blue). Scale bar = 50 µm. h Percentage of enteroids with CCK staining in G14, G2D12, RSP, AS, AS→RSP, and AS→RASP treatments. Average results are from three different enteroid lines or passages. ***p = 0.0002 (RSP to AS), 0.0001 (AS to AS→RSP); ****p < 0.0001. Bars show mean ± SEM; one-way ANOVA with Tukey correction for multiple comparisons (b, d, f, h). Tables above graphs show the total number of enteroids examined per condition. Each experiment repeated with at least three different enteroid lines. Source data are provided as a Source Data file.