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. 2022 Jan 11;13:261. doi: 10.1038/s41467-021-27901-5

Fig. 7. Differentiation Conditions Induce Hormone Secretion.

Fig. 7

a Serotonin (5HT) ELISA of conditioned media from the last two days of differentiation of enteroids grown in G14, G2D12, RSP, AS, AS→RSP, and AS→RASP. Representative experiment showing n = 3 wells from each condition from a single enteroid line. *p = 0.049; **p = 0.0049 (RSP to AS→RSP), 0.0011 (AS to AS→RSP); ***p = 0.0005 (G14 to AS→RSP and G2D12 to AS→RSP); ****p < 0.0001. b 5HT ELISA of AS conditioned media collected after 24 h on day 13 (solid bar) and after 24 h with forskolin (Fsk) on day 14 (striped bar). Representative experiment showing n = 3 wells from each condition from a single enteroid line. **p = 0.0015. c Glucose-dependent insulinotropic peptide (GIP) ELISA of conditioned media from the last two days of differentiation of enteroids grown in G14, G2D12, RSP, AS, AS→RSP, and AS→RASP. Representative experiment showing n = 3 wells from each condition from a single enteroid line. **p = 0.0013 (RSP to AS), 0.0031 (RSP to AS→RSP); ***p = 0.0009 (G14 to RSP, G2D12 to RSP, and RSP to AS→RASP); ****p < 0.0001. d GIP ELISA of AS→RSP conditioned media collected after 24 h on day 13 (solid bar) and after 24 h with Fsk on day 14 (striped bar). Representative experiment showing n = 3 wells from each condition from a single enteroid line. **p = 0.0025. Bars show mean ± SEM; one-way ANOVA with Tukey correction for multiple comparisons (a, c); two-tailed unpaired t test (b, d). Dotted line at 2.6 ng/mL represents the lower limit of detection for the 5HT ELISA (a, b). Dotted line at 4.2 pg/mL represents the lower limit of detection for the GIP ELISA (c, d). Each experiment repeated with at least three different enteroid lines. Source data are provided as a Source Data file.