Fig. 6. Metabolic mechanism of DGTS accumulation under −P conditions.

Changes in the contents of DGTS (white), PC (gray), and PE (black) in cells grown for 24 h after a shift from +P to −P conditions, which were estimated in cells on an OD₇₃₀ L culture basis (a), and in the culture (b). c Effects of metabolic inhibitors or light conditions on the accumulation of DGTS in the cells at 24 h after the shift to −P conditions. The values were estimated as the proportion of the DGTS content in the cells with application of chloramphenicol (CAP), cycloheximide (CHI), cerulenin, and DCMU, and in those shifted to the dark conditions, relative to that in non-treated control cells. The values shown are averages for two technical replicates for each of two biological replicates (a, b), or averages ± SEM for three biological replicates (c). Some dots are overlapped (refer to source data in Supplementary Data 1). Data in (c) were analyzed by one-way ANOVA with multiple comparison by Tukey–Kramer’s test.