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. 2022 Jan 11;12(1):5. doi: 10.1038/s41408-021-00603-3

Fig. 2. Treatment with Menin inhibitor SNDX-50469 depletes MEIS1, FLT3, CDK6, and BCL2 with concomitant induction of MCL1 and CD11b expression and features of morphologic differentiation in AML cells.

Fig. 2

A, B MOLM13 and OCI-AML3 cells were treated with the indicated concentrations of SNDX-50469 for 48 h. Following this, total cell lysates were prepared and immunoblot analyses were conducted. The expression levels of β-Actin in the lysates served as the loading control. C, D MOLM13 and OCI-AML3 cells were treated with the indicated concentrations of SNDX-50469 for 96 h or 7 days. Morphologic differentiation (% myelocytes, meta-myelocytes, or bands) was determined by light microscopy. Mean of three experiments ±S.E.M. ***p < 0.005; ****p < 0.001 (determined by two-tailed, unpaired t test in GraphPad V8). E MV4-11, OCI-AML3, and MOLM13 cells were treated with the indicated concentrations of SNDX-50469 or SNDX-5613 for 96 h. At the end of treatment, the % of TO-PRO-3 iodide-positive, non-viable cells were determined by flow cytometry. Columns, mean of three experiments ±S.E.M. F Kaplan–Meier survival curve of mice infused with MLL-AF9 + FLT3-TKD expressing PDX (AML#4) cells and treated with SNDX-5613 as indicated for 4 weeks.