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. 2022 Jan 11;13:245. doi: 10.1038/s41467-021-27921-1

Fig. 8. Ectopic expression of MAPK4 reduces human TNBC cell sensitivity to PI3K inhibitor treatments.

Fig. 8

Representative images and quantification of colony formation assay data of engineered a SUM159, b MDA-MB-468, c HCC1395, and d HCC1806 cells with 0.5 µg/ml Dox-induced ectopic expression of MAPK4 (iMAPK4) or control (iCtrl). The cells were also treated with increasing doses of Pictilisib, Alpelisib, or control. The right panels show quantification of colonies formed under each treatment condition described in the left panels. Data are mean ± SD. Adjusted P values determined by two-way ANOVA followed by Sidak’s multiple comparisons. e Western blots on SUM159 and HCC1806 cells with 0.5 µg/ml Dox-induced ectopic expression of MAPK4 (iMAPK4) or control (iCtrl) cells after 10 days culturing in the presence of Alpelisib (+) or DMSO (−). Data are representative of at least three independent experiments. Source data are provided as a Source data file.