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. 2022 Jan 11;13:243. doi: 10.1038/s41467-021-27335-z

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 1 — a Schematic of 9-day in vitro differentiation protocol employed to model cell-state transitions in axial elongation and sequential Hox cluster activation. D = day. b Quantitative PCR analysis of trunk (Hoxb8, Hoxc8) and posterior (Hoxd13) Hox genes during in vitro differentiation of wild-type (WT) and miR-196-triple knockout (miR-196-TKO) induced pluripotent stem cells (iPSCs). Data points (coloured squares) represent the mean of technical triplicate and biological duplicate analysis (error bar = SEM).