Table 1.

Clonal outgrowth frequency of T cell clones.

Gene delivery method	Frequency of top clone	Cumulative frequency of top 10 clones
TcB-M	0.05% ± 0.02%	0.41% ± 0.17%
PiggyBac	2.52% ± 1.74%	9.12% ± 2.83%
Sleeping Beauty	2.58% ± 1.37%	13.80% ± 7.76%
Lentivirus (Wang)	0.50% ± 0.25%	0.96% ± 0.34%
Lentivirus (Brady)	2.17% ± 0.56%	12.96% ± 1.98%

Summary table of the frequency of the top 10 T cell clones and the respective percentages of those clones with the T cell population, following different gene delivery conditions. TcB transposon system was tested and compared with lentiviral transduction datasets^32,33, as well as PiggyBac and Sleeping Beauty transposon systems³⁴. Table displays mean ± SD, biologically independent samples. n = 8 for TcB-M, n = 9 for SB, n = 9 for PiggyBac, n = 2 for LV (Wang), n = 4 for LV (Brady). Source data are provided as a Source Data file. Sequencing data used to perform integration site analysis are available online (Accession ID: PRJNA779430).