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. 2022 Jan 11;12:491. doi: 10.1038/s41598-021-04498-9

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Detection of KEMV Seg-2 and TBEV envelope genes by real-time RT-PCR in lysates of individual Ixodes ricinus after infection using the artificial feeding system—EL: Engorged Larvae, N: nymphs (N infected at the larval stage) and comparison of mean Ct values for each group (Kruskal–Wallis test, alpha = 5%, *: p value < 0.001). The lower and upper lines represent the lower (Q1) and upper (Q3) quartiles. The median is represented in the diagram and data falling outside the Q1-Q3 range are plotted as outliers of the data (circle).