. 2022 Jan 11;12:491. doi: 10.1038/s41598-021-04498-9

Table 1.

Engorgement rates of larvae and TBEV infection in engorged larvae and nymphs infected at larval stage after artificial feeding (AFS) or immersion techniques (khi-2 test, alpha = 5%, *: p value < 0.001).

Tested parameters	Control ticks fed on clean blood using AFS	Ticks fed on TBEV-spiked blood using AFS	Ticks immersed in TBEV suspension followed by feeding on CD1 mice
% of engorged larvae	23.16% (2085/9000)	22.32% (2009/9000)	78.32%* (224/286)
% of infected larvae	0% 0/20	70% (14/20)	72% (18/25)
% of infected nymphs	0% 0/20	75% (15/20)	48%* (39/82)

Tested parameters

Control ticks fed on clean blood using AFS

Ticks fed on TBEV-spiked blood using AFS

Ticks immersed in TBEV suspension followed by feeding on CD1 mice

% of engorged larvae

23.16%

(2085/9000)

22.32%

(2009/9000)

78.32%*

(224/286)

% of infected larvae

0/20

70%

(14/20)

72%

(18/25)

% of infected nymphs

0/20

75%

(15/20)

48%*

(39/82)