. 2022 Jan 11;12:491. doi: 10.1038/s41598-021-04498-9

Table 2.

Engorgement rates of larvae and KEMV infection in engorged larvae and nymphs infected at larval stage after artificial feeding (AFS) or immersion techniques (khi-2 test, alpha = 5%, *: p value < 0.001).

Tested parameters	Control ticks fed on clean blood using AFS	Ticks fed on KEMV-spiked blood using AFS	Ticks immersed in KEMV suspension followed by feeding on BALB/C mice
% of engorged larvae	56% (1120/2000)	29%* (581/2000)	73% (886/1200)
% of infected larvae	0% 0/30	83.3% 25/30	26,25% (21/80)*
% of infected nymphs	0% 0/10	40% 4/10	0* 0/25

Tested parameters

Control ticks fed on clean blood using AFS

Ticks fed on KEMV-spiked blood using AFS

Ticks immersed in KEMV suspension followed by feeding on BALB/C mice

% of engorged larvae

56%

(1120/2000)

29%*

(581/2000)

73%

(886/1200)

% of infected larvae

0/30

83.3%

25/30

26,25%

(21/80)*

% of infected nymphs

0/10

40%

4/10

0/25