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Experimental design of virus transmission from nymphs infected at the larval stage to a vertebrate host.
| Methods used to infect larval stages | Virus | Infestation date after moulting (months) | Number of mice | Nymphs per mouse | Organ sampling | Blood sampling | End (days post-infestation) | ||
|---|---|---|---|---|---|---|---|---|---|
| IFNAR−/− | BALB/c | CD1 | |||||||
| Artificial feeding | TBEV | 12 | 1 | – | – | 2 | Yes | Yes | 6 |
| KEMV | 5 | 4 | 4 | – | 30 | Yes | Yes | 10 | |
| Immersion | TBEV | 3–12 | – | – | 14 | 10 | No | No | 30 |
| KEMV | 5 | 4 | 4 | – | 30 | Yes | Yes | 10 | |
