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. 2022 Jan 11;5:41. doi: 10.1038/s42003-021-02934-0

Fig. 5. GALNT3 is overexpressed in Fucosylated cell lines and contributes to its glyco-phenotype.

Fig. 5

a Volcano plot of a differential gene expressed genes between the Lewishigh and Lewislow cell lines defined in. b Scheme of the GALNT3 enzymatic activity. c Expression of GALNT3 in the different tumour clusters in scRNA-seq. d Expression of Tn antigen (mAb: 83D4) and Sialyl-Tn antigen (mAb: B72.3) was analyzed by flow cytometry. e Western blot of GALNT3 in selected PDAC cell lines. f Confocal microscopy of GALNT3 (green) and actin (red). g Expression of GALNT3 is associated with the Fucosylated subtype in PDAC tissue. h Analysis of the expression of glycan structures using plant lectins and antibodies by flow cytometry in WT and GALNT3 KO cell lines. Statistical analysis: Two-way ANOVA with Sidak method for multiple test correction (*p ≤ 0.05; **p ≤ 0.01; ***p ≤ 0.001). i Western Blot of GALNT3, E-Cadherin and Actin in WT and GALNT3 KO cell lines.