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. 2022 Jan 11;13:224. doi: 10.1038/s41467-021-27934-w

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 7 — Volcano plots of differential gene expression obtained from RNA-seq of iWAT (a) and eWAT (b) after GIP analog exposure. iWAT RNA-seq data were analyzed using GSEA to evaluate changes in fatty-acid metabolism (c), oxidative phosphorylation (d), mitochondrial fatty-acid beta-oxidation (e) and adipogenesis (f). eWAT RNA-seq data were also analyzed for changes in fatty-acid metabolism (g) and oxidative phosphorylation (h). NES (normalized enrichment score) and nominal p value are depicted.