Fig. 5. Analysis of mouse strains with deletions of evolutionarily conserved domains of the Vsx2 SE.

A Drawing of the eight strains with deletions made using CRISPR-Cas9. There were three separate lines used for the consensus that had each site mutated individually and the combination of the two sites mutated. B Photograph of adult mice with each homozygous deletion and the orJ mouse strain as a reference for microphthalmia. C Photograph of eyes for each strain showing microphthalmia for the orJ, R0-37, and R0-37–R1-28 strains. D DAPI stained micrographs of retina from each strain. E Bar plot of mean with SEM of photopic vision (cycles/degree). n = 3 biologically independent mice for each deletion mouse strain. n = 8 biologically independent animals for wild-type mice. Abbreviations: ONL, outer nuclear layer; INL, inner nuclear layer; GCL, ganglion cell layer; Scale bars: 2.5 mm. Source data are provided as a Source Data file.