Skip to main content
. 2022 Jan 11;13:253. doi: 10.1038/s41467-021-27924-y

Fig. 6. Separation of functional regions of the Vsx2 SE.

Fig. 6

A, B qRT-PCR and RNA-seq of genes for retinal cell types for each of the indicated strains. Bipolar cells are missing from the original SE deletion strain and the R3-17 strain while there is a defect in retinal ganglion cell formation in the R1-28 deletion. C Principal component analysis (PCA) of bulk RNA-seq for each deletion mouse strain. The three consensus binding mutants are stacked on top of each other and indistinguishable from WT. D Micrographs showing the expression of Vsx2 in bipolar cells and Müller glia in the adult wild type and R1-28 deletion strain. The retinal thickness is about half that of the wild type but most cell types are present. E UMAP plots for WT, R1-28, CRC-SE, R3-17, R0-37, and orJ retinae. Stack bar plots show the percentage of each cell type below each UMAP. F Bar plot of the percentage of bipolar neurons in each of the retinae shown in (E). Abbreviations: ONL, outer nuclear layer; INL, inner nuclear layer; GCL, ganglion cell layer; PC1, PC2, principal components 1 and 2. Scale bars: 25 μm.