Fig. 3.

SHP-1 interacts with STING to inhibit the activation of STING. Luciferase assay of IFN-β (A) and ISRE (B) activation in HEK293T cells expressing various vectors (n = 3). C, D Immunoassay of lysates of HEK293T cells expressing various vectors. E Direct binding of GST-STING to His–SHP-1 (left) or to endogenous SHP-1 from peritoneal macrophages (right). F–I Immunoassay of lysates of HEK293T cells expressing various vectors. J Immunoassay of lysates of peritoneal macrophages from WT or me^v/me^v mice infected with HSV-1. The data are representative of at least three independent experiments. The data are the means ± SEMs. **P < 0.01 (two-tailed unpaired Student’s t test)