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. 2021 Nov 22;19(1):108–121. doi: 10.1038/s41423-021-00802-9

Fig. 5.

Fig. 5

Vif facilitates SHP-1-mediated inhibition of STING. A Immunoassay of lysates of HEK293T cells expressing various vectors. B Endogenous interaction of SHP-1 and STING in THP-1 cells infected with HIV-1 or HIV-1ΔVif. C–E Immunoassay of lysates from HEK293T cells expressing various vectors. Luciferase assays of IFN-β (F) and ISRE (G) activation in HEK293T cells expressing the indicated vectors (n = 3). H Immunoassay of lysates of MDMs transfected with SHP-1 or control shRNA and infected with HIV-1 or HIV-1ΔVif. I Immunoassay of lysates of THP-1 cells transfected with SHP-1 or control shRNA and infected with HIV-1 or HIV-1ΔVif. J Immunoblot (native PAGE) demonstrating STING dimerization in THP-1 cells transfected with SHP-1 or control shRNA and infected with HIV-1 or HIV-1ΔVif. K IFNB, CXCL10, ISG15, and TNF mRNA levels in THP-1 cells transfected with the indicated vectors and infected with HSV-1 for 12 h (n = 3). The data are representative of at least three independent experiments. The data are the means ± SEMs. *P < 0.05, **P < 0.01, and ***P < 0.001 (two-tailed unpaired Student’s t test)