Table 1.
Hormonal profile of cv. ‘Krezus’ embryos.
| Compound [pmol/g] DW | Function | Haploid embryo | Zygotic embryo | Zygotic embryo after 2,4-D treatment |
|---|---|---|---|---|
| Auxins | ||||
| IBA | Precursor | 298.7 ± 37.2 a | 66.9 ± 6.0 b | 83.5 ± 11.0 b |
| IAA | Active | 502 ± 10.3 b | 3059.8 ± 369.0 a | 2247.8 ± 648.1 a |
| 13CA | Inactivated | 18,501.3 ± 2164.3 a | 294.6 ± 12.9 b | 714.8 ± 287.5 b |
| MeIAA | Inactivated | 481.4 ± 83.1 a | 167.5 ± 38.1 a | 351.5 ± 166.1 a |
| IAA-Glu | Inactivated | 695.2 ± 69.5 a | 346.0 ± 14.1 b | 306.0 ± 11.5 b |
| IAA-Asp | Inactivated | 616 ± 29.4 a | 1007.6 ± 208.2 a | 918.2 ± 228.7 a |
| OxIAA | Inactivated | 232.7 ± 77.1 a | 81.5 ± 34.1 a | 127.7 ± 64.7 a |
| Sum of inactivated forms | 20,526.6 | 1897.2 | 2418.2 | |
| Cytokinins | ||||
| K | nd | nd | nd | |
| K-R | nd | nd | nd | |
| IPD | Precursor | 3.6 ± 0.7 a | 2.0 ± 0.4 a | 2.4 ± 0.3 a |
| IP | Precursor | 320.2 ± 29.3 a | 178.1 ± 13.6 b | 138.0 ± 14.5 b |
| Sum of precursors | 323.8 | 180.1 | 140.4 | |
| DH-Z | Active | 102.6 ± 31.6 a | 28.9 ± 10.3 b | 14.0 ± 7.0 b |
| c-Z | Active | 18.3 ± 5.5 a | 38.3 ± 10.0 b | 32.6 ± 7.1 a |
| t-Z | Active | 98.4 ± 24.7 a | 16.5 ± 3.5 b | 20.0 ± 2.8 b |
| DH-Z-R | Active | 8.8 ± 5.6 | nd | nd |
| c-Z-R | Active | 54.4 ± 5.4 a | 23.7 ± 3.5 b | 18.8 ± 8.2 b |
| t-Z-R | Active | 4.6 ± 2.3 a | 5.0 ± 0.5 a | 2.4 ± 0.5 a |
| Sum of active forms | 287.1 | 112.4 | 87.8 | |
| c-Z-O–G | Inactivated | 8.9 ± 4.8 b | 86.4 ± 9.3 a | 38.7 ± 12.7 b |
| c-Z-7-G | Inactivated | 31.5 ± 3.0 a | 7.6 ± 0.6 b | 17.3 ± 9.9 ab |
| t-Z-O–G | Inactivated | 114 ± 13.9 a | 42.2 ± 1.5 b | 32.3 ± 3.9 b |
| t-Z-7-G | Inactivated | 19.3 ± 1.2 a | 4.3 ± 2.2 b | 6.4 ± 1.1 b |
| Sum of inactivated forms | 173.7 | 140.5 | 94.7 | |
| Gibberellins | ||||
| GA9 | Precursor | 54.1 ± 54.1 a | 79.8 ± 12.4 a | 52.8 ± 4.8 a |
| GA5 | Precursor | 43.1 ± 22.6 a | 18.2 ± 9.3 a | 3.0 ± 3.0 a |
| Sum of precursors | 97.2 | 98 | 55.8 | |
| GA7 | Active | nd | nd | nd |
| GA4 | Active | nd | 6.4 ± 3.7 a | 4.3 ± 4.3 a |
| GA6 | Active | 214.0 ± 96.0 a | 96.4 ± 28.0 a | 114.1 ± 36.5 a |
| GA1 | Active | 61.7 ± 31.0 a | 30.4 ± 15.9 a | nd |
| GA3 | Active | 707.6 ± 328.8 a | 195.5 ± 85.0 a | 207.4 ± 70.0 a |
| Sum of active forms | 983.3 | 328.8 | 325.7 | |
| GA8 | Inactivated | 544.8 ± 379.8 a | 98.2 ± 24.9 a | 196.3 ± 26.5 a |
Values are means of three replicates ± standard error. Means indicated by the same letter do not significantly differ at P < 0.05 according to one-way ANOVA and post hoc Duncan’s test.