Table 2.
Hormonal profile of cv. ‘Akt’ embryos.
| Compound [pmol/g] DW | Function | Haploid embryo | Zygotic embryo | Zygotic embryo after 2,4-D treatment |
|---|---|---|---|---|
| Auxins | ||||
| IBA | Precursor | 517.8 ± 111.5 a | 51.0 ± 12.0 b | 50.9 ± 23.1 b |
| IAA | Active | 807.3 ± 23.3 a | 1814.4 ± 690.6 a | 2718.5 ± 1150.6 a |
| 13CA | Inactivated | nd | 705.8 ± 30.7 a | 720.5 ± 28.1 a |
| MeIAA | Inactivated | 916.6 ± 213.2 a | 186.0 ± 82.0 b | 317.5 ± 107.0 b |
| IAA-Glu | Inactivated | 1302.3 ± 178.5 a | 255.9 ± 65.3 b | 259.9 ± 36.1 b |
| IAA-Asp | Inactivated | 3052.1 ± 991.4 a | 1092.1 ± 347.4 a | 1314.0 ± 122.6 a |
| OxIAA | Inactivated | 299.7 ± 32.8 a | 169.7 ± 15.8 a | 316.0 ± 132.9 a |
| Sum of inactivated forms | 5570.7 | 2409.5 | 2927.9 | |
| Cytokinins | ||||
| K | nd | nd | nd | |
| K-R | nd | nd | nd | |
| IPD | Precursor | 1.2 ± 1.04 a | 0.7 ± 0.5 a | 1.0 ± 0.2 a |
| IP | Precursor | 586.9 ± 97.4 a | 43.7 ± 22.0 b | 92.8 ± 15.7 b |
| Sum of precursors | 588.1 | 44.4 | 93.8 | |
| DH-Z | Active | 153.4 ± 66.6 a | 10.2 ± 8.2 b | 6.8 ± 4.5 b |
| c-Z | Active | 61.4 ± 3.6 a | 26.4 ± 1.3 b | 24.1 ± 6.0 b |
| t-Z | Active | 130.8 ± 52.0 a | 26.2 ± 6.7 ab | 18.1 ± 4.6 b |
| DH-Z-R | Active | nd | nd | nd |
| c-Z-R | Active | 70.2 ± 2.5 a | 9.9 ± 1.8 b | 9.0 ± 2.0 b |
| t-Z-R | Active | 25.1 ± 0.3 a | 2.9 ± 0.4 b | 3.1 ± 0.3 b |
| Sum of active forms | 287.1 | 75.6 | 61.1 | |
| c-Z-O–G | Inactivated | nd | 17.1 ± 12.2 a | 26.6 ± 5.7 a |
| c-Z-7-G | Inactivated | 31.3 ± 15.6 a | 6.0 ± 1.7 a | 5.7 ± 0.8 a |
| t-Z-O–G | Inactivated | 185.2 ± 15.4 a | 28.2 ± 11.3 b | 26.2 ± 3.6 b |
| t-Z-7-G | Inactivated | 33.0 ± 6.6 a | 3.0 ± 1.6 b | 6.3 ± 1.4 b |
| Sum of inactivated forms | 249.5 | 54.3 | 64.8 | |
| Gibberellins | ||||
| GA9 | Precursor | 314.7 ± 49.4 a | 35.1 ± 21.7 b | 39.6 ± 4.0 b |
| GA5 | Precursor | 7.9 ± 7.9 | nd | nd |
| Sum of precursors | 322.7 | 35.1 | 39.6 | |
| GA7 | Active | nd | nd | 40.8 ± 22.6 |
| GA4 | Active | 113.4 ± 113.4 a | nd | 108.9 ± 60.8 a |
| GA6 | Active | 477.4 ± 67.8 a | 85.0 ± 37.0 b | 101.5 ± 32.3 b |
| GA1 | Active | nd | nd | 26.7 ± 16.8 |
| GA3 | Active | 1157.5 ± 221.8 a | nd | 169.8 ± 96.2b |
| Sum of active forms | 2192.5 | 258.2 | 588.1 | |
| GA8 | Inactivated | 444.1 ± 340.0 a | 173.2 ± 89.4 a | 140.4 ± 84.9a |
Values are means of three replicates ± standard error. Means indicated by the same letter do not significantly differ at P < 0.05 according to one-way ANOVA and post hoc Duncan’s test.