Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2022 Jan 11;13:218. doi: 10.1038/s41467-021-27896-z

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Fig. 5 — a An illustration showing the experimental design for rescuing EDAC on stiff ECM and the effect of different mosaic populations on extrusion of transformed cells on stiff ECM. b–e Scatter bar plots depicting the fraction of GFP-HRas^V12 expressing colonies extruded over substrates of varying stiffness. For each stiffness, left bars are for mock MDCK-WT: MDCK-HRas^V12GFP and right bars are for mApple-dnFLNA-MDCK:MDCK-GFP-HRas^V12 (b), MDCK-mApple-dnFAM101B:MDCK-GFP-HRas^V12 (c), mApple-dnNesprin1-MDCK:MDCK-GFP-HRas^V12 (d) or mApple-dnLaminB1-MDCK:MDCK-GFP-HRas^V12 (e) mosaic populations. Stable expression of dnFLNA, dnFAM101B, dnNesprin1 or dnLaminB1 in surrounding cells rescued extrusion of transformed populations on the stiff substrate. The number of colonies counted is indicated inside each bar. Data are mean ± s.e.m. collected over three independent biological replicates. Statistical significance was assessed using Unpaired t-test with Welch’s correction (two-tailed). Significance value (when p < 0.0001) p = 9.27612e−09. Source data (b–e) are provided as a Source Data file. f Summary of the integrative mechanism that connects the processes occurring at different length scales. We elucidate matrix mechanics, a microenvironmental factor (a), influences the molecular dynamics of filamin (b and c) to determine the outcome of EDAC (d).