Figure 4.

miR-874-3p targets Adam19 during vascular calcification (VC)

(A) Schematic describing the identification of mRNA targets for miR-874-3p. (B) miR-874-3p/Adam19 binding sites. (C) The expression level of Adam19 in RNA-seq. (D) Verification of Adam19 expression using qualitative real-time PCR (n = 4). (E) Expression of Adam19 after adding a miR-873-3p mimic with or without Pi treatment. Cells were treated with 4 mM inorganic phosphate (Pi) for 2 days post-transfection. (F) Calcium concentration (mg Ca/mg protein) after Adam19 inhibition (n = 4). Cells were treated with 4 mM Pi and then cultured for an additional 2 days post-transfection. Data represent mean ± SEM. Statistical significance was determined using Student’s t test (∗p ≤ 0.05, ∗∗p ≤ 0.01, and ∗∗∗p ≤0.001).