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0.2% NP-40 buffer
| Reagent | Final concentration | Amount |
|---|---|---|
| Tris-HCl (1 M), pH 7.4 | 25 mM | 25 mL |
| NaCl (4 M) | 150 mM | 37.5 mL |
| Glycerol | 10% | 100 mL |
| NP-40 | 0.2% | 2 mL |
| ddH2O | n/a | 835.5 mL |
| Total | n/a | 1 L |
Can be stored at 4°C for up to 3 months. Protease Inhibitor Cocktail (100×) will be added before use.
