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6× loading buffer
| Reagent | Final concentration | Amount |
|---|---|---|
| Tris-HCl (0.5M), pH 6.8 | 60 mM | 24 mL |
| SDS | 12% | 24 g |
| Bromophenol blue | 0.06% | 120 mg |
| DTT | 600 mM | 18.6 g |
| Glycerol | 49% | 98 mL |
| ddH2O | n/a | 42 mL |
| Total | n/a | 200 mL |
Can be store at -20°C for up to one year, and can be diluted accordingly.
