Fig. 2.

UCA1 is an hnRNP I/L binding partner. A, B, No effects of UCA1 on hnRNP I/L expression in BLCA cells. HnRNP I/L expression was analyzed in UCA1 knockdown and UCA1 overexpression BLCA cells by qRT-PCR (A) and western blot (B). C to F, Correlation between hnRNP I/L and UCA1 expression in BLCA cells. UCA1 expression was analyzed by qRT-PCR (C and E). The expression of hnRNP I/L was analyzed by qRT-PCR (C and E) and western blot (D and F). HnRNP I/L deletion decreases the expression of UCA1 in BLCA cells; Ectopic hnRNP I/L increases the expression of UCA1 in BLCA cells. The expression of hnRNP I or L do not affect the expression of the other counterpart. *P < 0.01. G, H, RNA pulldown assays validation of UCA1-hnRNP I/L binding regions in 5637 cells. Biotinylated full-length UCA1 (UCA1-FL) probe or truncated UCA1 probes (UCA1–1 and UCA1–2) were incubated with 5637 cell lysates. HnRNP I/L in samples pulled down by streptavidin beads were analyzed using western blot (G). Two forms of UCA1–1 mutated at the hnRNP I /L peak binding regions were described. HnRNP I/L in samples pulled down by wild type UCA1–1 and UCA1–1 mt probes labeled with biotin were analyzed by western blot (H).