Table 2.
qRCR primers (5′->3′) | |||||
UCA1 | Forward | CTCTCCATTGGGTTCACCATTC | |||
Reverse | GCGGCAGGTCTTAAGAGATGAG | ||||
hnRNP I | Forward | AATGACAAGAGCCGTGACTAC | |||
Reverse | GGAAACCAGCTCCTGCATAC | ||||
hnRNP L | Forward | TTGTGGCCCTGTCCAGAGAATT | |||
Reverse | GTTTGTGTAGTCCCAAGTATCCTG | ||||
SLC1A5 | Forward | GACCGTACGGAGTCGAGAAG | |||
Reverse | GGGGGTTTCCTTCCTCAGTG | ||||
SLC7A5 | Forward | GAAGGCACCAAACTGGATGT | |||
Reverse | GAAGTAGGCCAGGTTGGTCA | ||||
GLS2 | Forward | TGCCTATAGTGGCGATGTCTCA | |||
Reverse | GTTCCATATCCATGGCTGACAA | ||||
GPT2 | Forward | GGAGCTAGTGACGG CATTTCTACGA | |||
GPT2 promotor | Reverse | CCCAGGGTTGATTATGCAGAGCA | |||
Forward | CCTCCCCTGTCCCTACTGAT | ||||
Reverse | ATGTCCATGCAGTCCCTTGC | ||||
β-actin | Forward | TCCCTGGAGAAGAGCTACGA | |||
Reverse | AGCACTGTGTTGGCGTACAG | ||||
GAPDH | Forward | GTCGGAGTCAACGGATTTG | |||
Reverse | TGGGTGGAATCATATTGGAA | ||||
shRNA | |||||
UCA1 | CCGGGTTAATCCAGGAGACAAAGACTCGAGTCTTTGTCTCCTGGATTAACTTTTTG | ||||
hnRNP I | CCGGGCGTGAAGATCCTGTTCAATACTCGAGTATTGAACAGGATCTTCACGCTTTTTG | ||||
hnRNP L | CCGGCCTCAACAACAACTTCATGTTCTCGAGAACATGAAGTTGTTGTTGAGGTTTTTG | ||||
GPT2 | CCGGGACAACGTGTACTCTCCAGATCTCGAGATCTGGAGAGTACACGTTGTCTTTTTG | ||||
Control | CCGGTTCTCCGAACGTGTCACGTCTCGAGACGTGACACGTTCGGAGAATTTTTG | ||||
Anti-sense probes for selective retrieval of UCA1 by CHIRP | |||||
Probe 1 | aagggttgtaggttgtttgt | ||||
Probe 2 | cggcaggtcttaagagatga | ||||
Probe 3 | gttttagacttttgacccag | ||||
Probe 4 | atatgcgtgtactgttgtcc | ||||
Probe 5 | ccaagccctctaacaacaaa | ||||
Probe 6 | gcagatcctatgcagaagag | ||||
Probe 7 | aatagtattccctgttgcta | ||||
Probe 8 | aatgtaggtggcgatgagtt |