Table 2.
Cell biomass and lipid content of Y. lipolytica WT and gsy1Δ-LPAAT2 strains cultured on 20 g L−1 glycerol, glucose, or palm oil or on 10 g L−1 glucose +10 g L−1 palm oil in nitrogen-limited media. The values are means ± SD of measurements made on three separate cultures for each genotype. * denote values significantly (P < 0.05) different from WT (Student's t-tests) and # from glycerol (ANOVA + Tukey HSD test).
| Strain | Substrate | Cell biomass (g L−1) | Lipid content (% of CDW) | Lipid titre (g L−1) |
|---|---|---|---|---|
| WT | Glycerol | 4.0 ±0.1 | 11.5 ±0.5 | 0.5 ±0.1 |
| gsy1Δ-LPAAT2 | 3.3 ±0.2* | 21.5 ±0.9* | 0.7 ±0.1* | |
| WT | Glucose | 4.0 ±0.1 | 10.1 ±1.8 | 0.4 ±0.1# |
| gsy1Δ-LPAAT2 | 3.7 ±0.1*# | 17.0 ±1.7*# | 0.6 ±0.1*# | |
| WT | Palm oil | 6.3 ±1.1# | 48.0 ±6.5# | 3.1 ±0.8# |
| gsy1Δ-LPAAT2 | 6.7 ±1.3*# | 46.6 ±0.4# | 3.1 ±0.5# | |
| WT | Glucose + | 5.9 ±0.2# | 42.4 ±2.2# | 2.5 ±0.1# |
| gsy1Δ-LPAAT2 | Palm oil | 5.9 ±1.3# | 46.8. ±2.1* | 2.8 ±0.5# |