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. 2021 Dec 7;298(1):101470. doi: 10.1016/j.jbc.2021.101470

Figure 8.

Figure 8

Magnified views of both motifs and membrane-embedded models of LPLAT9 and LPLAT12.A, magnified views of AGPAT Motifs 1 to 4 in LPLAT9 and PlsC (PDB 5KYM) (28) and MBOAT Motifs B to D in LPLAT12 and DGAT1 (PDB 6VP0) (42). For LPLAT9 and PlsC, AGPAT Motifs 1 to 4 are colored green, magenta, blue, and orange, respectively. For LPLAT12, MBOAT Motifs B to D are colored magenta, blue, and orange, respectively. For DGAT1, C18:1-CoA is shown in yellow bound to the model, with Motifs B and C of DGAT1 colored magenta and blue, respectively. B, membrane-embedded model of LPLAT9 and LPLAT12 was prepared as in Figure 7. Acetyl-CoA and lyso-PAF were manually incorporated into LPLAT9, and arachidonoyl-CoA and lyso-PC were manually incorporated into LPLAT12. Surface model represents the protein surface for LPLAT9 and the cavity surface for LPLAT12. Array of spheres represents membrane surface. Note that the membrane orientation of the LPLAT12, with arachidonoyl-CoA entering from the cytosol and lyso-PC entering from the luminal side of the membrane, is opposite of a recently proposed model by Zhang et al. (43) (discussed more in main text).