. Author manuscript; available in PMC: 2022 Dec 14.

Published in final edited form as: Anal Chem. 2021 Dec 4;93(49):16664–16672. doi: 10.1021/acs.analchem.1c04225

Table 1.

Kinetics parameters of 24A, B, C phosphorylation by recombinant EGFR and Akt, and reporter lifetime in A431 lysates (All values are n = 3 for 24A, B, C, *p ≤ 0.0486).

Reporter Structure			Akt Kinetics		EGFR Kinetics		A431 Lysate
Name	Linker	EGFR Site	K_M (μM)	k_cat (s⁻¹)	K_M (μM)	k_cat (s⁻¹)	Half-Life (min)
24A	rrr	Y	14 ± 3	0.7 ± 0.1	82 ± 22*	7.7 ± 1.2	267 ± 26
24B	Ahx	Y	28 ± 16	1.0 ± 0.4	812 ± 459*	19.8 ± 10.2	222 ± 21
24C	rrr	L-Htc	17 ± 5	0.8 ± 0.1	365 ± 201	15.0 ± 6.8	248 ± 23