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Analysis of p53 UFMylation modification in cells
HEK293T cells were transiently transfected by expression plasmids of UFMylation system components and Flag-tagged p53. Cell lysates were immunoprecipitated with anti-Flag M2 Affinity Gel and UFMylation of p53 was analysed by western blot with anti-UFM1 antibody.
Data is reproduced from Liu et al. (2020).
