| Tube A |
Tube B |
||||
|---|---|---|---|---|---|
| Reagents | Amount | Reagents | Amount | Ratios of DNAs | |
| Step 1 | pSG5-HA-UBA5 | 2 μg | 1 | ||
| pSG5-HA-UFC1 | 2 μg | 1 | |||
| PEI solution | 54 μL | pSG5-HA-UFL1 | 2 μg | 1 | |
| pSG5-HA-DDRGK1 | 2 μg | 1 | |||
| pSG5-HA-UFM1 | 6 μg | 3 | |||
| pCDNA-FLAG-His-p53 | 2–6 μg | 1–3 | |||
| Opti-MEM | 500 μL | Opti-MEM | 500 μL | ||
| Step 2 | Mix diluted DNA of tube B to diluted PEI of tube A | ||||
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