| Reagent | Final concentration | Suggested ratios of DNAs | Amount |
|---|---|---|---|
| ATP (10 mM) | 5 mM | N/A | 10 μL |
| UFL1 (100 ng/μL) | 5 ng/μL | 1 | 1 μL |
| DDRGK1 (200 ng/μL) | 5 ng/μL | 1 | 0.5 μL |
| UBA5 (250 ng/μL) | 5 ng/μL | 1 | 0.5 μL |
| UFC1 (1 μg/μL) | 5 ng/μL | 1 | 0.2 μL |
| UFM1 (2.5 μg/μL) | 5 ng/μL | 1 | 0.5 μL |
| MgCl2 (100 mM) | 10 mM | N/A | 2 μL |
| Substrate protein (700 ng/μL) | 17.5 ng/μL | 3.5 | 0.5 μL |
| PBS-X | N/A | N/A | 4.8 μL |
| Total | N/A | N/A | 20 μL |
Official websites use .gov
A
.gov website belongs to an official
government organization in the United States.
Secure .gov websites use HTTPS
A lock (
) or https:// means you've safely
connected to the .gov website. Share sensitive
information only on official, secure websites.