Lysis buffer
| Reagent | Final concentration | Amount |
|---|---|---|
| pH8.0 Tris-HCl (1 M stock) | 150 mM | 15 mL |
| SDS | 5% (w/v) | 5 g |
| Glycerol (100% stock) | 30% | 30 mL |
| ddH2O | N/A | 55 mL |
| Total | N/A | 100 mL |
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