Figure 4.

Effects of the AAV-PHP.eB-SaCas9-KKH-g2 virus on cell survival rates in Myo6^WT/C442Y mice

(A and B) The number of IHCs (A) and OHCs (B) per 100-μm section for four untreated and four AAV-PHP.eB-SaCas9-KKH-g2-treated Myo6^WT/C442Y cochleae from four mice at 5 months. (C and D) The numbers of IHCs (C) and OHCs (D) per 100-μm section for three untreated and three AAV-PHP.eB-SaCas9-KKH-g2-treated Myo6^WT/C442Y cochleae from three mice at 10 months. Values and error bars reflect the mean ± SEM. Statistical analysis was by two-tailed Student’s t tests. ∗∗∗p < 0.001. NS, not significant. (E) Representative confocal images of cochleae harvested at 10 months from the AAV-PHP.eB-SaCas9-KKH-g2-treated and untreated ears of Myo6^WT/C442Y mice. The apical, middle, and basal turns were dissected and stained with MYO7A (red) for hair cells and with DAPI (blue) for nuclei. Scale bar, 50 μm.