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Cargo-based delivery methods of the CRISPR-Cas9 complex
Strategy I: plasmid-based CRISPR-Cas9 system encoding both Cas9 and sgRNA. Strategy II: cellular delivery of Cas9 encoding mRNA in combination with sgRNA. Inside the cell, the mRNA is translated to Cas9 protein, and the Cas9/sgRNA complex is formed. Strategy III: direct delivery of the fully assembled Cas9/sgRNA complex into the cell.29
