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CPP-mediated delivery of Cas9/sgRNA system
(A) Cas9 protein conjugated to a CPP (m9R) via a thioether bond. (B) Subsequently, sgRNA forms a complex with the CPP domain of CPP(m9R) due to electrostatic attractions.64 Amino acids indicated by yellow (Cys), brown (Gly), blue (Arg), and orange (Lys).
