Figure 1.

Expansion and characterization of UABCs

(A and B) UABCs cultured in tissue-cultured plates coated with iMatrix (recombinant laminin 511) showed improved proliferation (A) immediately after plating from tissue and (B) during subsequent passages. Error bars indicate standard deviation. (C) Telomerase activity in the expanded UABCs was measured using telomeric repeat amplification protocol (TRAP), and telomerase activity was observed in UABCs across multiple passages. (D and E) Significant shortening of telomere lengths was also not observed in expanded UABCs when telomere lengths were probed using (D) Southern blot or (E) qPCR.