Figure 2.

Correction of the pathogenic mutation with truncated sgRNA

(A and B) sgRNAs used in ABEmax-NG group (A) or Sc-ABEmax group (B). The target sites are highlighted in red and with a numeric subscript. The non-target sites are highlighted in blue and with a numeric subscript. The PAM sequences are underlined. (C and D) The correction efficiency was calculated for the target and non-target sites in the ABEmax-NG group (C) or Sc-ABEmax group (D) with different truncated sgRNAs. Data are shown as mean ± SEM (n = 3 from three independent experiments).