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. 2021 May 14;30(1):164–174. doi: 10.1016/j.ymthe.2021.05.010

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Off-target alteration and indel profile of Nme2Cas9 with hTTR G3 gRNA in mouse and human genomes

(A) The indel ratio of on-target and predicted off-target sites in the liver genomes of mouse livers that received AAV8-Nme2Cas9-G3 (hTTR G3) treatment or NT gRNA treatment (NT gRNA, n = 4; hTTR G3, n = 4), and HEK293T cells transfected with Nme2Cas9 with G3 gRNA or NT gRNA revealed by targeted deep sequencing. (B) The indel profile induced by Nme2Cas9 with hTTR G3 gRNA in the genomes of mouse livers and HEK293T cells. Frequencies of the top 5 were analyzed. +, insertion; –, deletion.