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Small molecule inducible systems to control Cas9 expression
(A) The intein-mediated Cas981 has Cas9 fused to intein sequences. Once 4-OHT is added, the inteins are spliced out, leaving a functional Cas9. (B) The iCas system82 consists of Cas9 fused to the hormone-binding domain of the estrogen receptor (ERT2). Upon addition of 4-OHT, which binds to ERT2, Cas9 is translocated to the nucleus to partake in gene-editing activity.
